Simplified Fluorescence Microscopy of Tubercle Bacilli

Simplified Fluorescence Microscopy of Tubercle Bacilli

The fluorescence of tubercle bacilli stained with auramine and rhodamine does not require ultra-violet light. It can be caused by blue light up to 406mp. A’copper sdphate ammonia liquid filter, suitably diluted, will transmit this waveband. A gelatin screen filter, absorbing all light below 510mp. is used in the eyepiece. Normal, high intensity projection Alament lamps, combined with lamp conde...

Examination of smears for tubercle bacilli by fluorescence microscopy.

I n underdeveloped countries, laboratory faci l i ties for the bacterio­ logical d iagnosis of tubercu losis are, at present , very l imited . Cultural methods are un l i kely to be used on a large scale for many years to come. It is , therefore, important to investigate the most economical method of examin ing smears for tubercle baci l l i . Fluorescence microscopy was introduced by H agemann...

The development of fluorescence microscopy for tubercle bacilli and its use as an adjunct to histological routine.

The microscopic demonstration of the tubercle baciilus by the Ziehl-Neelsen technique has always entailed heavy demands on the time and concentration of the observer, but in recent years fluorescence microscopy has offered a means of greatly facilitating this task. The spontaneous fluorescence of M. tuberculosis under ultra-violet microscopy was first observed by Kaiserling (1917), but the use ...

DIFFERENTIATION OF LEPROSY AND TUBERCLE BACILLI. 219 On the Differentiation of Leprosy and Tubercle Bacilli

On the nucleoproteins of avian tubercle bacilli.

The nucleic acids of tubercle bacilli have formed the subject of several studies, among which may be mentioned those by Ruppel (l), Levene (2), and Johnson and his associates (3-5). The work of Mensel and Heidelberger (6) on the fractionation of the proteins of the tubercle bacillus revealed the presence of several protein fractions, rich in phosphorus and in purines, which appeared to be nucle...